Semen viability is reduced during freezing and subsequent thawing. If the dog and bitch are both alive and if natural mating or insemination with fresh semen can be arranged, this is likely to result in better fertility than inseminating with frozen semen.
The best time to freeze semen is when the dog is young and highly fertile. Do not wait until the dog is getting on in age and his fertility is declining. Even fertile dogs' semen may have deteriorated in quality sufficiently for it not to be freezable.
How much semen can be frozen per attempt depends on body size and underlying fertility of the dog, and can only be determined for each dog by means of a complete semen evaluation, a prerequisite before semen is frozen. More than one ejaculate may be required to freeze sufficient semen to inseminate a bitch in very small dogs or in dogs with low sperm counts.
Freshly-produced semen freezes best. It is therefore wise to freeze semen within a week after the dog has last been used for a mating.
After freezing a dose is thawed and evaluated. If the quality is acceptable, it must be transferred to a long-term storage facility.